Journal: Scientific Reports

Article Title: P-Selectin preserves immune tolerance in mice and is reduced in human cutaneous lupus

doi: 10.1038/srep41841

Figure Lengend Snippet: ( a) Relative frequency of peripheral blood leukocyte populations of 1.5–3 month-old WT and P-Sel −/− mice. ( b , d ) Percentage of IL-10 + conventional dendritic cells (cDC), plasmacytoid DC (pDC), monocytes, granulocytes and B cells; and frequency of IL-10 and IL-17 producing CD4 + and CD8 + T lymphocytes, in 1.5-month-old ( b ) and >18-month-old ( d ) WT and P-Sel −/− mice. ( c) Representative dot plots of IL-10 + cDCs and IL-10 and IL-17 producing CD4 + T cells in 1.5-months old WT and P-Sel −/− mice. ( e) Phenotyping of CD4 + and CD8 + splenic T lymphocytes according to the expression of the naïve/memory/effector markers CD62L and CD44 in 1.5–3 months-old (upper panels) and >18 month-old (lower panels) WT and P-Sel −/− mice. ( f) Representative dot plots showing the distribution of 1.5–3-month-old mice splenic populations according to the expression of L-Selectin and CD44. In all cases, n = 4 mice per group. In all cases, n = 4 mice per group. Bars represent the mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.005, by Student’s two tailed t test.

Article Snippet: Consecutive tissue sections of sun-exposed skin biopsies underwent immunohistochemistry with the Dako REAL EnVision Detection System Peroxidase/DAB+kit (Dako), using as primary antibodies anti-human P-Selectin (10 μg/ml) (R&D Systems; Minneapolis, MN, USA) and anti-human CD31 (1/50) (Abcam).

Techniques: Expressing, Two Tailed Test

Journal: Scientific Reports

Article Title: P-Selectin preserves immune tolerance in mice and is reduced in human cutaneous lupus

doi: 10.1038/srep41841

Figure Lengend Snippet: ( a) Representative photomicrographs (20×) of anti-CD31 and anti-P-Selectin stained skin biopsies of healthy donors and SLE patients (upper panels). Black arrows point blood vessels. ( b) 200% magnification of representative blood vessels from the original images are represented (lower panels). ( c) Classification and quantification of CD31+ dermal blood vessels according to the expression level of P-Selectin (healthy controls, n = 4; SLE patients, n = 4); bars show the mean ± SD. **p < 0.01 by Student’s two tailed t test.

Article Snippet: Consecutive tissue sections of sun-exposed skin biopsies underwent immunohistochemistry with the Dako REAL EnVision Detection System Peroxidase/DAB+kit (Dako), using as primary antibodies anti-human P-Selectin (10 μg/ml) (R&D Systems; Minneapolis, MN, USA) and anti-human CD31 (1/50) (Abcam).

Techniques: Staining, Expressing, Two Tailed Test

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: The role of kinesin, dynein and microtubules in pancreatic secretion

doi: 10.1007/s00018-009-0052-0

Figure Lengend Snippet: Immunoelectron microscopic localization of kinesin, dynein, and tubulin in resting acinar cells. Ultrathin sections of pancreatic tissue were incubated either with anti-kinesin and anti-tubulin antibodies (a–c, g), or with anti-dynein and anti-tubulin antibodies (d–f) followed by 6 and 12 nm gold-conjugated secondary antibodies (see “Materials and methods”). Lumen of acinar cells (L) and zymogen granules (ZG) are marked. Boxes denote regions of higher magnification shown in b, c, e, f. Neither dynein nor kinesin can be detected in the apical cellular region (c, f) or at the surface of zymogen granules (b, e, g). Tubulin, detected with 6 nm gold particles colocalizes with kinesin and dynein in the perinuclear region (b, e), and can additionally be found near the apical lumen of acinar cells (c, f). Images shown are representative for at least five experiments. Bars 5 μm (a, d), 1 μm (g) or 0.25 μm (b, c, e, f)

Article Snippet: The antibodies (Ab) used were as follows: goat anti-mouse IgG Cy™3-labeled Ab (Jackson Immuno Research, West Grove, PA, USA), goat anti-mouse Biotin-conjugated IgG (Vector Laboratories), goat anti-mouse IgG horseradish peroxidase conjugated (Sigma), goat anti-mouse biotin-SP-conjugated IgG (Jackson Immuno Research), goat anti-mouse IgG labeled with 12 nm colloidal gold (Dianova), goat anti-rabbit IgG labeled with 6 nm colloidal gold (Dianova), goat anti-rabbit Alexa-594 labeled IgG (Molecular Probes, Eugene, OR, USA), mouse monoclonal antibody (mAb) raised against Golgi 58 K protein/formiminotransferase cyclodeaminase (FTCD) (Sigma), anti-dynein mAb cytoplasmic 74 kD intermediate chains (Chemicon International, Temecula, CA, USA), mAb raised against kinesin heavy chain (Chemicon International), anti-β-tubulin mAb (Sigma), mouse IgG (Dianova), rabbit polyclonal antibody (Ab) raised against tubulin (Sigma), sheep FITC-conjugated anti-digoxigenin Ab (Boehringer Mannheim, Germany).

Techniques: Incubation